I beleive there is limit today of 30 bots in a league file (and 30 challengers at a time). This limit appears pretty arbitrary, mostly just the way the code was originally written due to limits on the league dialog UI. There is no fundenmental reason why this limit can't be increased. I will add this to my to-do list.
On a different note, I would like to see a league where hand-coding is entirely prohibited. That is, a league where the only requirement is that the bot be 100% evolved from an all-zero DNA first-bot predecesor. People can use whatever means they wish to breed their entry over as many cycles and in whatever environments they wish provided none of the bot's DNA has it's orgins in a hand-coded organism (thus, no viral gene transfer from hand-coded bots in the breeding sim).